Using Two-Photon Intravital Imaging to Study Developmental Plasticity of Neural Circuits
نویسندگان
چکیده
Laser-scanning two-photon microscopy offers well-known advantages for imaging deeper into living tissue, but even in relatively translucent organisms like the zebrafish or albino Xenopus tadpole it can be a powerful tool for studying brain development [1]. The visual system in the Xenopus tadpole utilizes both molecular guidance and activity-dependent cues to form appropriate connections [2]. N-methyl-Daspartate (NMDA) type glutatmate receptors have been shown to participate in the activity-dependent mechanisms of circuit formation, however their specific contributions to the process of axonal projection structural refinement is not fully understood.
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